RNA
Part:BBa_K3356019
Designed by: Luke Weir Group: iGEM20_Nottingham (2020-10-27)
Synthetic guide RNA sequence 1 for tetR integration upstream of spoIIIAA
Sequence coding for synthetic guide RNA 1 to direct the cas9 mediated insertion of the TetR inducible promoter upstream of the spoIIIA gene. This is to create a conditional sporulating strain which will only sporulate in the presence of the inducer, anhydrotetracyline (aTc), which is a derivative of tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |